publications

Research

TREM-1 is required for enhanced OpZ-induced superoxide generation following priming.

Murthy S, Baruah S, Bowen JL, Keck K, Wagner BA, Buettner GR, Sykes DB, Klesney-Tait J.

J Leukoc Biol. 2022 Jan 24. doi: 10.1002/JLB.3A0421-212R. PMID: 35075692.

Transfusable neutrophil progenitors as cellular therapy for the prevention of invasive fungal infections.

Sykes DB, Martinelli MM, Negoro P, Xu S, Maxcy K, Timmer K, Viens AL, Alexander NJ, Atallah J, Snarr BD, Baistrocchi SR, Atallah NJ, Hopke A, Scherer A, Rosales I, Irimia D, Sheppard DC, Mansour MK.

J Leukoc Biol. 2022 Jun;111(6):1133-1145. doi: 10.1002/JLB.4HI1221-722R. Epub 2022 Mar 31. PMID: 35355310.

Disruption of dNTPs Homeostasis by Ribonucleotide Reductase Hyperactivation Overcomes AML Differentiation Blockade.

Wang H, He X, Zhang L, Dong H, Huang F, Xian J, Li M, Chen W, Lu X, Pathak KV, Huang W, Li Z, Zhang L, Nguyen LXT, Yang L, Feng L, Gordon D, Zhang J, Pirrotte P, Chen CW, Salhotra A, Kuo YH, Horne DA, Marcucci G, Sykes DB, Tiziani S, Jin H, Wang X, Li L.

Blood. 2022 Apr 19:blood.2021015108. doi: 10.1182/blood.2021015108. PMID: 35439288

TLR Signaling Rescues Fungicidal Activity in Syk-Deficient Neutrophils.

Viens AL, Timmer KD, Alexander NJ, Barghout R, Milosevic J, Hopke A, Atallah NJ, Scherer AK, Sykes DB, Irimia D, Mansour MK.

J Immunol. 2022 Apr 1;208(7):1664-1674. doi: 10.4049/jimmunol.2100599. PMID: 35277418

A new murine model of Barth syndrome neutropenia links TAFAZZIN deficiency to increased ER stress-induced apoptosis.

Sohn J, Milosevic J, Brouse T, Aziz N, Elkhoury J, Wang S, Hauschild A, van Gastel N, Cetinbas M, Tufa SF, Keene DR, Sadreyev RI, Pu WT, Sykes DB.

Blood Adv. 2022 Apr 26;6(8):2557-2577. doi: 10.1182/bloodadvances.2021005720. PMID: 34979560.

Regulation of chromatin accessibility by the histone chaperone CAF-1 sustains lineage fidelity.

Franklin R, Guo Y, He S, Chen M, Ji F, Zhou X, Frankhouser D, Do BT, Chiem C, Jang M, Blanco MA, Vander Heiden MG, Rockne RC, Ninova M, Sykes DB, Hochedlinger K, Lu R, Sadreyev RI, Murn J, Volk A, Cheloufi S.

Nat Commun. 2022 Apr 29;13(1):2350. doi: 10.1038/s41467-022-29730-6. PMID: 35487911.

Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes.

Ko J, Wilkovitsch M, Oh J, Kohler RH, Bolli E, Pittet MJ, Vinegoni C, Sykes DB, Mikula H, Weissleder R, Carlson JCT.

Nat Biotechnol. 2022 Jun 2. doi: 10.1038/s41587-022-01339-6. PMID: 35654978.

In vivo genome-wide CRISPR screening in murine acute myeloid leukemia uncovers microenvironmental dependencies.

Mercier FE, Shi J, Sykes DB, Oki T, Jankovic M, Man CH, Kfoury YS, Miller E, He S, Zhu A, Vasic R, Doench J, Orthwein A, Michor F, Scadden DT.

Blood Adv. 2022 Jul 6:bloodadvances.2022007250. doi: 10.1182/bloodadvances.2022007250. PMID: 35793392.

A transcriptional metastatic signature predicts survival in clear cell renal cell carcinoma.

Alchahin AM, Mei S, Tsea I, Hirz T, Kfoury Y, Dahl D, Wu CL, Subtelny AO, Wu S, Scadden DT, Shin JH, Saylor PJ, Sykes DB, Kharchenko PV, Baryawno N.

Nat Commun. 2022 Sep 30;13(1):5747. doi: 10.1038/s41467-022-33375-w. PMID: 36180422; PMCID: PMC9525645.

Image-seq: spatially resolved single-cell sequencing guided by in situ and in vivo imaging.

Haase C, Gustafsson K, Mei S, Yeh SC, Richter D, Milosevic J, Turcotte R, Kharchenko PV, Sykes DB, Scadden DT, Lin CP.

Nat Methods. 2022 Dec;19(12):1622-1633. doi: 10.1038/s41592-022-01673-2. Epub 2022 Nov 24. PMID: 36424441; PMCID: PMC9718684.

ENT1 blockade by CNX-774 overcomes resistance to DHODH inhibition in pancreatic cancer.

Mullen NJ, Thakur R, Shukla SK, Chaika NV, Kollala SS, Wang D, He C, Fujii Y, Sharma S, Mulder SE, Sykes DB, Singh PK.

Cancer Lett. 2023 Jan 1;552:215981. doi: 10.1016/j.canlet.2022.215981. Epub 2022 Oct 27. PMID: 36341997.

mTORC1 links pathology in experimental models of Still’s disease and macrophage activation syndrome.

Huang Z, You X, Chen L, Du Y, Brodeur K, Jee H, Wang Q, Linder G, Darbousset R, Cunin P, Chang MH, Wactor A, Wauford BM, Todd MJC, Wei K, Li Y, Levescot A, Iwakura Y, Pascual V, Baldwin NE, Quartier P, Li T, Gianatasio MT, Hasserjian RP, Henderson LA, Sykes DB, Mellins ED, Canna SW, Charles JF, Nigrovic PA, Lee PY.

Nat Commun. 2022 Nov 28;13(1):6915. doi: 10.1038/s41467-022-34480-6. PMID: 36443301; PMCID: PMC9705324.

Inhibiting the biogenesis of myeloid-derived suppressor cells enhances immunotherapy efficacy against mammary tumor progression.

Colligan SH, Amitrano AM, Zollo RA, Peresie J, Kramer ED, Morreale B, Barbi J, Singh PK, Yu H, Wang J, Opyrchal M, Sykes DB, Nemeth MJ, Abrams SI.

J Clin Invest. 2022 Dec 1;132(23):e158661. doi: 10.1172/JCI158661. PMID: 36453551.

Neutrophil profiles of pediatric COVID-19 and multisystem inflammatory syndrome in children.

Boribong BP, LaSalle TJ, Bartsch YC, Ellett F, Loiselle ME, Davis JP, Gonye ALK, Sykes DB, Hajizadeh S, Kreuzer J, Pillai S, Haas W, Edlow AG, Fasano A, Alter G, Irimia D, Sade-Feldman M, Yonker LM.

Cell Rep Med. 2022 Dec 20;3(12):100848. doi: 10.1016/j.xcrm.2022.100848. Epub 2022 Nov 21.

tiRNA signaling via stress-regulated vesicle transfer in the hematopoietic niche.

Kfoury YS, Ji F, Mazzola M, Sykes DB, Scherer AK, Anselmo A, Akiyama Y, Mercier F, Severe N, Kokkaliaris KD, Zhao T, Brouse T, Saez B, Seidl J, Papazian A, Ivanov P, Mansour MK, Sadreyev RI, Scadden DT.

Cell Stem Cell. 2021 Dec 2;28(12):2090-2103.e9. doi: 10.1016/j.stem.2021.08.014. Epub 2021 Sep 21.

KAT6A and ENL form an epigenetic transcriptional control module to drive critical leukemogenic gene expression programs.

Yan F, Li J, Milosevic J, Petroni R, Liu S, Shi Z, Yuan S, Reynaga JM, Qi Y, Rico J, Yu S, Liu Y, Rokudai S, Palmisiano N, Meyer SE, Sung PJ, Wan L, Lan F, Garcia BA, Stanger BZ, Sykes DB, Blanco MA.

Cancer Discov. 2021 Dec 1:candisc.1459.2020. doi: 10.1158/2159-8290.CD-20-1459. Online ahead of print.

Chromatin-state barriers enforce an irreversible mammalian cell fate decision.

Blanco MA, Sykes DB, Gu L, Wu M, Petroni R, Karnik R, Wawer M, Rico J, Li H, Jacobus WD, Jambhekar A, Cheloufi S, Meissner A, Hochedlinger K, Scadden DT, Shi Y.

Cell Rep. 2021 Nov 9;37(6):109967. doi: 10.1016/j.celrep.2021.109967.

Human prostate cancer bone metastases have an actionable immunosuppressive microenvironment.

Kfoury Y, Baryawno N, Severe N, Mei S, Gustafsson K, Hirz T, Brouse T, Scadden EW, Igolkina AA, Kokkaliaris K, Choi BD, Barkas N, Randolph MA, Shin JH, Saylor PJ, Scadden DT, Sykes DB, Kharchenko PV; as part of the Boston Bone Metastases Consortium.

Cancer Cell. 2021 Nov 8;39(11):1464-1478.e8. doi: 10.1016/j.ccell.2021.09.005. Epub 2021 Oct 15.

Systematic tissue collection during clinical breast biopsy is feasible, safe and enables high-content translational analyses.

Koh SB, Dontchos BN, Bossuyt V, Edmonds C, Cristea S, Melkonjan N, Mortensen L, Ma A, Beyerlin K, Denault E, Niehoff E, Hirz T, Sykes DB, Michor F, Specht M, Lehman C, Ellisen LW, Spring LM.

NPJ Precis Oncol. 2021 Sep 21;5(1):85. doi: 10.1038/s41698-021-00224-w.

A Subset of Localized Prostate Cancer Displays an Immunogenic Phenotype Associated with Losses of Key Tumor Suppressor Genes.

Calagua C, Ficial M, Jansen CS, Hirz T, Del Balzo L, Wilkinson S, Lake R, Ku AT, Voznesensky O, Sykes DB, Saylor PJ, Ye H, Signoretti S, Kissick H, Sowalsky AG, Balk SP, Einstein DJ.

Clin Cancer Res. 2021 Sep 1;27(17):4836-4847. doi: 10.1158/1078-0432.CCR-21-0121. Epub 2021 Jun 24.

Host defense against fungal pathogens: Adaptable neutrophil responses and the promise of therapeutic opportunities?

Scherer AK, Hopke A, Sykes DB, Irimia D, Mansour MK.

PLoS Pathog. 2021 Jul 29;17(7):e1009691. doi: 10.1371/journal.ppat.1009691. eCollection 2021 Jul.

Neutrophils require SKAP2 for reactive oxygen species production following C-type lectin and Candida stimulation.

Nguyen GT, Xu S, Adams W, Leong JM, Bunnell SC, Mansour MK, Sykes DB, Mecsas J.

iScience. 2021 Jul 16;24(8):102871. doi: 10.1016/j.isci.2021.102871. eCollection 2021 Aug 20.

Combined epigenetic and metabolic treatments overcome differentiation blockade in acute myeloid leukemia.

Zee BM, Poels KE, Yao CH, Kawabata KC, Wu G, Duy C, Jacobus WD, Senior E, Endress JE, Jambhekar A, Lovitch SB, Ma J, Dhall A, Harris IS, Blanco MA, Sykes DB, Licht JD, Weinstock DM, Melnick A, Haigis MC, Michor F, Shi Y.

iScience. 2021 May 25;24(6):102651. doi: 10.1016/j.isci.2021.102651. eCollection 2021 Jun 25.

Induction of a Timed Metabolic Collapse to Overcome Cancer Chemoresistance.

van Gastel N, Spinelli JB, Sharda A, Schajnovitz A, Baryawno N, Rhee C, Oki T, Grace E, Soled HJ, Milosevic J,Sykes DB, Hsu PP, Vander Heiden MG, Vidoudez C, Trauger SA, Haigis MC, Scadden DT.

Cell Metab. 2020 Sep 1;32(3):391-403.e6. doi: 10.1016/j.cmet.2020.07.009. Epub 2020 Aug 6.

Aldehyde dehydrogenase 3a2 protects AML cells from oxidative death and the synthetic lethality of ferroptosis inducers.

Yusuf R, Saez B, Sharda A, van Gastel N, Yu V, Baryawno N, Scadden EW, Acharya SS, Chattophadhyay S, Huang C, Viswanathan V, S’aulis D, Cobert J,Sykes DB, Keibler MA, Das S, Hutchinson JN, Churchill M, Mukherjee S, Lee D, Mercier FE, Doench J, Bullinger L, Logan DJ, Schreiber S, Stephanopoulos G, Rizzo WB, Scadden DT.

Blood. 2020 May 26:blood.2019001808. doi: 10.1182/blood.2019001808. Online ahead of print.

Spleen Tyrosine Kinase Is a Critical Regulator of Neutrophil Responses to Candida Species.

Negoro PE, Xu S, Dagher Z, Hopke A, Reedy JL, Feldman MB, Khan NS, Viens AL, Alexander NJ, Atallah NJ, Scherer AK, Dutko RA, Jeffery J, Kernien JF, Fites JS, Nett JE, Klein BS, Vyas JM, Irimia D,Sykes DB, Mansour MK.

mBio. 2020 May 12;11(3):e02043-19. doi: 10.1128/mBio.02043-19.

SKAP2 is required for defense against K. pneumoniae infection and neutrophil respiratory burst.

Nguyen GT, Shaban L, Mack M, Swanson KD, Bunnell SC,Sykes DB, Mecsas J.

Elife. 2020 Apr 30;9:e56656. doi: 10.7554/eLife.56656.

Chromatin accessibility promotes hematopoietic and leukemia stem cell activity.

Cabal-Hierro L, van Galen P, Prado MA, Higby KJ, Togami K, Mowery CT, Paulo JA, Xie Y, Cejas P, Furusawa T, Bustin M, Long HW, Sykes DB, Gygi SP, Finley D, Bernstein BE, Lane AA.

Nat Commun. 2020 Mar 16;11(1):1406. doi: 10.1038/s41467-020-15221-z.

Glycerol-3-phosphate is an FGF23 regulator derived from the injured kidney.

Simic P, Kim W, Zhou W, Pierce KA, Chang W, Sykes DB, Aziz NB, Elmariah S, Ngo D, Pajevic PD, Govea N, Kestenbaum BR, de Boer IH, Cheng Z, Christov M, Chun J, Leaf DE, Waikar SS, Tager AM, Gerszten RE, Thadhani RI, Clish CB, Jüppner H, Wein MN, Rhee EP.
J Clin Invest. 2020 Mar 2;130(3):1513-1526. doi: 10.1172/JCI131190.

A modern reassessment of glycoprotein-specific direct platelet autoantibody testing in immune thrombocytopenia.

Al-Samkari H, Rosovsky RP, Karp Leaf RS, Smith DB, Goodarzi K, Fogerty AE, Sykes DB, Kuter DJ.
Blood Adv. 2020 Jan 14;4(1):9-18. doi: 10.1182/bloodadvances.2019000868.

The novel dihydroorotate dehydrogenase (DHODH) inhibitor BAY 2402234 triggers differentiation and is effective in the treatment of myeloid malignancies.

Christian S, Merz C, Evans L, Gradl S, Seidel H, Friberg A, Eheim A, Lejeune P, Brzezinka K, Zimmermann K, Ferrara S, Meyer H, Lesche R, Stoeckigt D, Bauser M, Haegebarth A, Sykes DB, Scadden DT, Losman JA, Janzer A.
Leukemia. 2019 Oct;33(10):2403-2415. doi: 10.1038/s41375-019-0461-5. Epub 2019 Apr 2.

Frontline Science: Employing enzymatic treatment options for management of ocular biofilm-based infections.

Kugadas A, Geddes-McAlister J, Guy E, DiGiandomenico A, Sykes DB, Mansour MK, Mirchev R, Gadjeva M.
J Leukoc Biol. 2019 Jun;105(6):1099-1110. doi: 10.1002/JLB.4HI0918-364RR. Epub 2019 Jan 28.

A post-transcriptional program of chemoresistance by AU-rich elements and TTP in quiescent leukemic cells.

Lee S, Micalizzi D, Truesdell SS, Bukhari SIA, Boukhali M, Lombardi-Story J, Kato Y, Choo MK, Dey-Guha I, Ji F, Nicholson BT, Myers DT, Lee D, Mazzola MA, Raheja R, Langenbucher A, Haradhvala NJ, Lawrence MS, Gandhi R, Tiedje C, Diaz-Muñoz MD, Sweetser DA, Sadreyev R, Sykes D, Haas W, Haber DA, Maheswaran S, Vasudevan S.
Genome Biol. 2020 Feb 10;21(1):33. doi: 10.1186/s13059-020-1936-4.

Biguanides enhance antifungal activity against Candida glabrata.

Xu S, Feliu M, Lord AK, Lukason DP, Negoro PE, Khan NS, Dagher Z, Feldman MB, Reedy JL, Steiger SN, Tam JM, Soukas AA, Sykes DB, Mansour MK
Virulence. 2018;9(1):1150-1162

Fluorescent Tracking of Yeast Division Clarifies the Essential Role of Spleen Tyrosine Kinase in the Intracellular Control of Candida glabrata in Macrophages.

Dagher Z, Xu S, Negoro PE, Khan NS, Feldman MB, Reedy JL, Tam JM, Sykes DB, Mansour MK
Frontiers in Immunology. 2018 May 16;9:1058

An unappreciated role for neutrophil-DC hybrids in immunity to invasive fungal infections.

Fites JS, Gui M, Kernien JF, Negoro P, Dagher Z, Sykes DB, Nett JE, Mansour MK, Klein BS
PLOS Pathogens. 2018 May 21;14(5):e1007073

Amino acid-insensitive mTORC1 regulation enables nutritional stress resilience in hematopoietic stem cells.

Kalaitzidis D, Lee D, Efeyan A, Kfoury Y, Nayyar N, Sykes DB, Mercier FE, Papazian A, Baryawno N, Victora GD, Neuberg D, Sabatini DM, Scadden DT.
The Journal of clinical investigation. 2017; 127(4):1405-1413.

Bone marrow-derived immature myeloid cells are a main source of circulating suPAR contributing to proteinuric kidney disease.

Hahm E, Wei C, Fernandez I, Li J, Tardi NJ, Tracy M, Wadhwani S, Cao Y, Peev V, Zloza A, Lusciks J, Hayek SS, O’Connor C, Bitzer M, Gupta V, Sever S, Sykes DB, Scadden DT, Reiser J.
Nature medicine. 2017; 23(1):100-106. NIHMSID: NIHMS855786

Development of ML390: A Human DHODH Inhibitor That Induces Differentiation in Acute Myeloid Leukemia.

Lewis TA, Sykes DB, Law JM, Muñoz B, Rustiguel JK, Nonato MC, Scadden DT, Schreiber SL.
ACS medicinal chemistry letters. 2016; 7(12):1112-1117.

Inhibition of Dihydroorotate Dehydrogenase Overcomes Differentiation Blockade in Acute Myeloid Leukemia.

Sykes DB, Kfoury YS, Mercier FE, Wawer MJ, Law JM, Haynes MK, Lewis TA, Schajnovitz A, Jain E, Lee D, Meyer H, Pierce KA, Tolliday NJ, Waller A, Ferrara SJ, Eheim AL, Stoeckigt D, Maxcy KL, Cobert JM, Bachand J, Szekely BA, Mukherjee S, Sklar LA, Kotz JD, Clish CB, Sadreyev RI, Clemons PA, Janzer A, Schreiber SL, Scadden DT.
Cell. 2016; 167(1):171-186.e15.

Non-genotoxic conditioning for hematopoietic stem cell transplantation using a hematopoietic-cell-specific internalizing immunotoxin.

Palchaudhuri R, Saez B, Hoggatt J, Schajnovitz A, Sykes DB, Tate TA, Czechowicz A, Kfoury Y, Ruchika F, Rossi DJ, Verdine GL, Mansour MK, Scadden DT.
Nature biotechnology. 2016; 34(7):738-45. NIHMSID: NIHMS821329

Single Targeted Exon Mutation Creates a True Congenic Mouse for Competitive Hematopoietic Stem Cell Transplantation: The C57BL/6-CD45.1(STEM) Mouse.

Mercier FE, Sykes DB, Scadden DT.
Stem cell reports. 2016; 6(6):985-92.

Endogenous transmembrane protein UT2 inhibits pSTAT3 and suppresses hematological malignancy.

Lee D, Wang YH, Kalaitzidis D, Ramachandran J, Eda H, Sykes DB, Raje N, Scadden DT.
The Journal of clinical investigation. 2016; 126(4):1300-10.

RECQL5 Suppresses Oncogenic JAK2-Induced Replication Stress and Genomic Instability.

Chen E, Ahn JS, Sykes DB, Breyfogle LJ, Godfrey AL, Nangalia J, Ko A, DeAngelo DJ, Green AR, Mullally A.
Cell reports. 2015; 13(11):2345-52. NIHMSID: NIHMS739343

Control of signaling-mediated clearance of apoptotic cells by the tumor suppressor p53.

Yoon KW, Byun S, Kwon E, Hwang SY, Chu K, Hiraki M, Jo SH, Weins A, Hakroush S, Cebulla A, Sykes DB, Greka A, Mundel P, Fisher DE, Mandinova A, Lee SW.
Science (New York, N.Y.). 2015; 349(6247):1261669. NIHMSID: NIHMS794793

Neuregulin1-β decreases IL-1β-induced neutrophil adhesion to human brain microvascular endothelial cells.

Wu L, Walas S, Leung W, Sykes DB, Wu J, Lo EH, Lok J.
Translational stroke research. 2015; 6(2):116-24. NIHMSID: NIHMS599324

Niche-Based Screening in Multiple Myeloma Identifies a Kinesin-5 Inhibitor with Improved Selectivity over Hematopoietic Progenitors.

Chattopadhyay S, Stewart AL, Mukherjee S, Huang C, Hartwell KA, Miller PG, Subramanian R, Carmody LC, Yusuf RZ, Sykes DB, Paulk J, Vetere A, Vallet S, Santo L, Cirstea DD, Hideshima T, Dančík V, Majireck MM, Hussain MM, Singh S, Quiroz R, Iaconelli J, Karmacharya R, Tolliday NJ, Clemons PA, Moore MA, Stern AM, Shamji AF, Ebert BL, Golub TR, Raje NS, Scadden DT, Schreiber SL.
Cell reports. 2015; NIHMSID: NIHMS655802

Dectin-1 activation controls maturation of β-1,3-glucan-containing phagosomes.

Mansour MK, Tam JM, Khan NS, Seward M, Davids PJ, Puranam S, Sokolovska A, Sykes DB, Dagher Z, Becker C, Tanne A, Reedy JL, Stuart LM, Vyas JM.
The Journal of biological chemistry. 2013; 288(22):16043-54.

Putative RNA-splicing gene LUC7L2 on 7q34 represents a candidate gene in pathogenesis of myeloid malignancies.

Singh H, Lane AA, Correll M, Przychodzen B, Sykes DB, Stone RM, Ballen KK, Amrein PC, Maciejewski J, Attar EC.
Blood cancer journal. 2013; 3:e117.

SIRT1 regulates differentiation of mesenchymal stem cells by deacetylating β-catenin.

Simic P, Zainabadi K, Bell E, Sykes DB, Saez B, Lotinun S, Baron R, Scadden D, Schipani E, Guarente L.
EMBO molecular medicine. 2013; 5(3):430-40.

Discovering Small Molecules that Overcome Differentiation Arrest in Acute Myeloid Leukemia

Sykes DB, Haynes MK, Waller A, Garcia M, Ursu O, Gouveia KE, Sklar L, Lewis TA, Dandapani S, Munoz B, Scadden DT, Palmer M, Schreiber SL.
Bethesda (MD): National Center for Biotechnology Information (US); 2010. Chapter ml390

The Spt-Ada-Gcn5-acetyltransferase complex interaction motif of E2a is essential for a subset of transcriptional and oncogenic properties of E2a-Pbx1.

Scheele JS, Kolanczyk M, Gantert M, Zemojtel T, Dorn A, Sykes DB, Möbest DC, Kamps MP, Räpple D.
Leukemia & lymphoma. 2009; 50(5):816-28.

Quantitative expansion of ES cell-derived myeloid progenitors capable of differentiating into macrophages.

Odegaard JI, Vats D, Zhang L, Ricardo-Gonzalez R, Smith KL, Sykes DB, Kamps MP, Chawla A
Journal of leukocyte biology. 2007; 81(3):711-9. NIHMSID: NIHMS17132

Quantitative production of macrophages or neutrophils ex vivo using conditional Hoxb8.

Wang GG, Calvo KR, Pasillas MP, Sykes DB, Häcker H, Kamps MP.
Nature methods. 2006; 3(4):287-93.

E2a/Pbx1 induces the rapid proliferation of stem cell factor-dependent murine pro-T cells that cause acute T-lymphoid or myeloid leukemias in mice.

Sykes DB, Kamps MP.
Molecular and cellular biology. 2004; 24(3):1256-69.

Transcriptional profiling during the early differentiation of granulocyte and monocyte progenitors controlled by conditional versions of the E2a-Pbx1 oncoprotein.

Sykes DB, Scheele J, Pasillas M, Kamps MP.
Leukemia & lymphoma. 2003; 44(7):1187-99.

Estrogen-regulated conditional oncoproteins: tools to address open questions in normal myeloid cell function, normal myeloid differentiation, and the genetic basis of differentiation arrest in myeloid leukemia.

Sykes DB, Kamps MP.
Leukemia & lymphoma. 2003; 44(7):1131-9.

TH2 cytokines and allergic challenge induce Ym1 expression in macrophages by a STAT6-dependent mechanism.

Welch JS, Escoubet-Lozach L, Sykes DB, Liddiard K, Greaves DR, Glass CK.
The Journal of biological chemistry. 2002; 277(45):42821-9.

Nup98-HoxA9 immortalizes myeloid progenitors, enforces expression of Hoxa9, Hoxa7 and Meis1, and alters cytokine-specific responses in a manner similar to that induced by retroviral co-expression of Hoxa9 and Meis1.

Calvo KR, Sykes DB, Pasillas MP, Kamps MP.
Oncogene. 2002; 21(27):4247-56.

An induced Ets repressor complex regulates growth arrest during terminal macrophage differentiation.

Klappacher GW, Lunyak VV, Sykes DB, Sawka-Verhelle D, Sage J, Brard G, Ngo SD, Gangadharan D, Jacks T, Kamps MP, Rose DW, Rosenfeld MG, Glass CK.
Cell. 2002; 109(2):169-80.

Meis1a suppresses differentiation by G-CSF and promotes proliferation by SCF: potential mechanisms of cooperativity with Hoxa9 in myeloid leukemia.

Calvo KR, Knoepfler PS, Sykes DB, Pasillas MP, Kamps MP.
Proceedings of the National Academy of Sciences of the United States of America. 2001; 98(23):13120-5.

Estrogen-dependent E2a/Pbx1 myeloid cell lines exhibit conditional differentiation that can be arrested by other leukemic oncoproteins.

Sykes DB, Kamps MP.
Blood. 2001; 98(8):2308-18.

HoxB8 requires its Pbx-interaction motif to block differentiation of primary myeloid progenitors and of most cell line models of myeloid differentiation.

Knoepfler PS, Sykes DB, Pasillas M, Kamps MP.
Oncogene. 2001; 20(39):5440-8.

The HoxB1 hexapeptide is a prefolded domain: implications for the Pbx1/Hox interaction.

Slupsky CM, Sykes DB, Gay GL, Sykes BD.
Protein science : a publication of the Protein Society. 2001; 10(6):1244-53.

Hoxa9 immortalizes a granulocyte-macrophage colony-stimulating factor-dependent promyelocyte capable of biphenotypic differentiation to neutrophils or macrophages, independent of enforced meis expression.

Calvo KR, Sykes DB, Pasillas M, Kamps MP.
Molecular and cellular biology. 2000; 20(9):3274-85.

Granulocyte-macrophage colony-stimulating factor and B7-2 combination immunogene therapy in an allogeneic Hu-PBL-SCID/beige mouse-human glioblastoma multiforme model.

Parney IF, Petruk KC, Zhang C, Farr-Jones M, Sykes DB, Chang LJ.
Human gene therapy. 1997; 8(9):1073-85.

Tempi Syndrome

The TEMPI Syndrome.

Sykes DB, O’Connell C, Schroyens WA.
Blood. 2020 Feb 27. pii: blood.2019004216. doi: 10.1182/blood.2019004216

Complete Responses in the TEMPI Syndrome after Treatment with Daratumumab.

Sykes DB, Schroyens W
The New England Journal of Medicine. 2018 Jun 7;378(23):2240-2242

Long-term complete clinical and hematological responses of the TEMPI syndrome after autologous stem cell transplantation.

Kenderian SS, Rosado FG, Sykes DB, Hoyer JD, Lacy MQ.
Leukemia. 2015; 29(12):2414-6.

Difficulties in hematopoietic progenitor cell collection from a patient with TEMPI syndrome and severe iatrogenic iron deficiency.

Belizaire R, Sykes DB, Chen YB, Spitzer TR, Makar RS.
Transfusion. 2015; 55(9):2142-8.

Bone marrow findings of the newly described TEMPI syndrome: when erythrocytosis and plasma cell dyscrasia coexist.

Rosado FG, Oliveira JL, Sohani AR, Schroyens W, Sykes DB, Kenderian SS, Lacy MQ, Kyle RA, Hoyer JD.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc. 2015; 28(3):367-72.

Complete and partial responses of the TEMPI syndrome to bortezomib.

Schroyens W, O’Connell C, Sykes DB.
The New England journal of medicine. 2012; 367(8):778-80.

The TEMPI syndrome–a novel multisystem disease.

Sykes DB, Schroyens W, O’Connell C.
The New England journal of medicine. 2011; 365(5):475-7.

Case Reports

JAK inhibition in a patient with a STAT1 gain-of-function variant reveals STAT1 dysregulation as a common feature of aplastic anemia.

Rosenberg JM, Peters JM, Hughes T, Lareau CA, Ludwig LS, Massoth LR, Austin-Tse C, Rehm HL, Bryson B, Chen YB, Regev A, Shalek AK, Fortune SM, Sykes DB.

Med (N Y). 2022 Jan 14;3(1):42-57.e5. doi: 10.1016/j.medj.2021.12.003. PMID: 35590143

Severe autoimmune hemolytic anemia following receipt of SARS-CoV-2 mRNA vaccine.

Gadi SRV, Brunker PAR, Al-Samkari H, Sykes DB, Saff RR, Lo J, Bendapudi P, Leaf DE, Leaf RK.
Transfusion. 2021 Nov;61(11):3267-3271. doi: 10.1111/trf.16672. Epub 2021 Oct 3. PMID: 34549821

Severe babesiosis with associated splenic infarcts and asplenia.

Sporn ZA, Fenves AZ, Sykes DB, Al-Samkari H.

Proc (Bayl Univ Med Cent). 2021 Jun 1;34(5):597-599. doi: 10.1080/08998280.2021.1930632. eCollection 2021 Sep.

Severe autoimmune hemolytic anemia following receipt of SARS-CoV-2 mRNA vaccine.

Gadi SRV, Brunker PAR, Al-Samkari H, Sykes DB, Saff RR, Lo J, Bendapudi P, Leaf DE, Leaf RK.

Transfusion. 2021 Nov;61(11):3267-3271. doi: 10.1111/trf.16672. Epub 2021 Oct 3.

A case of antisynthetase syndrome with thrombotic thrombocytopenic purpura.

Goreshnik A, Serling-Boyd N, Theodore M, Champion S, Stemmer-Rachamimov A, Sykes DB.

Rheumatology (Oxford). 2021 Apr 6;60(4):e143-e145. doi: 10.1093/rheumatology/keaa717.PMID: 33221867 Free PMC article. No abstract available.

Case 10-2020: An 83-Year-Old Man with Pancytopenia and Acute Renal Failure.

Sykes DB, Zhang EW, Karp Leaf RS, Nardi V, Turbett SE.N Engl J Med. 2020 Mar 26;382(13):1258-1266. doi: 10.1056/NEJMcpc1916250.PMID: 32212524 No abstract available.

A cryptic imatinib-sensitive G3BP1-PDGFRB rearrangement in a myeloid neoplasm with eosinophilia.

Jan M, Grinshpun DE, Villalba JA, Dal Cin P, Sykes DB, Iafrate AJ, Ebert BL, Hobbs GS, Nardi V.
Blood Adv. 2020 Feb 11;4(3):445-448. doi: 10.1182/bloodadvances.2019001182.

A man with polycythemia vera, myelodysplastic syndrome and acquired microcytosis.

Mann M, Kreuzbauer T, Sykes DB.
BMJ Case Rep. 2019 Aug 13;12(8). pii: e229695. doi: 10.1136/bcr-2019-229695.

Immune neutropenia mediated by micafungin.

Ameri AH, Curtis BR, Sykes DB.
Am J Hematol. 2019 Jul;94(7):830-832. doi: 10.1002/ajh.25483. Epub 2019 Apr 14.

The Successful Use of Apixaban in Dialysis Patients with Calciphylaxis Who Require RAnticoagulation: A etrospective Analysis.

Garza-Mayers AC, Shah R, Sykes DB, Nigwekar SU, Kroshinsky D.
American Journal of Nephrology. 2018;48(3):168-171.

Acquired haemophilia A with a recalcitrant high-titre factor VIII inhibitor in the setting of interstitial lung disease.

Sun L, Sykes DB
BMJ Case Report. 2017 Jul 28;2017. pii: bcr-2017-220932.

Case 40-2017. A 32-Year-Old Woman with Headache, Abdominal Pain, Anemia, and Thrombocytopenia.

Sykes DB, Rosovsky RP, Singhal AB, Gonzalez RG, Moy AP.
The New England Journal of Medicine. 2017 Dec 28;377(26):2581-2590

Hormones and the bone marrow: panhypopituitarism and pancytopenia in a man with a pituitary adenoma.

Lang D, Mead JS, Sykes DB.
Journal of general internal medicine. 2015; 30(5):692-6.

Hyperhemolysis syndrome in a patient without a hemoglobinopathy, unresponsive to treatment with eculizumab.

Gupta S, Fenves A, Nance ST, Sykes DB, Dzik WS.
Transfusion. 2015; 55(3):623-8.

Case report: a 37-year-old male with telangiectasias, polycythemia vera, perinephric fluid collections, and intrapulmonary shunting.

Khan J, Sykes DB.
BMC hematology. 2014; 14(1):11.

Reviews

Host defense against fungal pathogens: Adaptable neutrophil responses and the promise of therapeutic opportunities?

Scherer AK, Hopke A, Sykes DB, Irimia D, Mansour MK.
PLoS Pathog. 2021 Jul 29;17(7):e1009691. doi: 10.1371/journal.ppat.1009691. eCollection 2021 Jul. PMID: 34324592.

The emergence of dihydroorotate dehydrogenase (DHODH) as a therapeutic target in acute myeloid leukemia.

David B. Sykes
Expert Opin Ther Targets. 2018 Nov;22(11):893-898.

Modulating Cell Fate as a Therapeutic Strategy.

Lin B, Srikanth P, Castle AC, Nigwekar S, Malhotra R, Galloway JL, Sykes DB, Rajagopal J.
Cell Stem Cell. 2018 Sep 6;23(3):329-341

The hematopoietic stem cell niche.

Park D, Sykes DB, Scadden DT.
Frontiers in bioscience (Landmark edition). 2012; 17:30-9. NIHMSID: NIHMS577418

Editorial

Auto-Phlebotomy.

Sykes DB.
Am J Med. 2022 Sep 29:S0002-9343(22)00714-8. doi: 10.1016/j.amjmed.2022.09.004. Epub ahead of print. PMID: 36181790.

The Art of Oncology: COVID Era.

Reynolds KL, Klempner SJ, Parikh A, Hochberg EP, Michaelson MD, Mooradian MJ, Lee RJ, Soumerai TE, Hobbs G, Piotrowska Z, Sykes DB, Farago AF, Bardia A, Rosovsky RP, Ryan DP.
Oncologist. 2020 Jul 22:10.1634/theoncologist.2020-0512. doi: 10.1634/theoncologist.2020-0512. Online ahead of print.